HPLC法测定水产品中阿苯达唑及其主要代谢物残留的研究

论文编号:SW080  论文字数:10084,页数:28

摘 要：本文主要阐述了应用高效液相色谱（HPLC）方法测定水产品中阿苯达唑（ABZ）及其两种主要代谢物砜（ABZSO2）、亚砜（ABZSO）残留量的研究。色谱条件是：色谱柱为ZORBAX-SB 5μm C18柱；流动相是酸化乙腈：水(25:75；v/v)；甲醇-磷酸缓冲液（75︰25；v/v），流速是1.0mL/min，柱温是30℃，选择的检测波长是295.4nm。采用甲醇：磷酸缓冲液=75：25定容系列标准液，阿苯达唑、阿苯达唑砜、阿苯达唑亚砜在0.1～10μg/mL范围内线性关系良好，加标浓度在100μg/kg～500μg/kg时，回收率分别ABZSO为83%～95%；ABZSO2为85%～97%；ABZ为70%～80%。研究结果显示，该方法的最低检测限、准确度和精密度均符合残留检测方法的要求。此检测分析方法处理样品快速、高效、准确，可作为水产品中阿苯达唑及其主要代谢物的有效检测方法。
关键词：水产品、高效液相色谱法、阿苯达唑及其代谢物

Determination of Albendazole and its metabolites in Aquatic products by HPLC
Abstract: The determination of Albendazole and its metabolites in aquatic products was established by using high performance liquid chromatography (Agilent-1100, USA) with a photodiode array detector detector (DAD, USA), a C18 column (ZORBAX-SB). The mobile phase was a mixture of acidified acetonitrile and water (25/75, v/v) and a mixture of methanol and phosphate buffer (75/25, v/v). 20 µl samples at room temperature injected onto the column through a 20 µl sample loop. Column temperature is 300C.The mobile phase rate was delivered at 1.0 ml/min. Detection wavelength: 295.4nm. In the range of 0. 1 - 10μg/ml, the results of Albendazole and its metabolite are linearly dependent. The mean extraction recoveries of Albendazole and its metabolites ranged from 80% to 90%. The method of determining the Albendazole and its metabolites in aquatic product was established by using reversed-phase HPLC, which had characters of simple and rapid operation, high yield and good reproducibility.

Keywords：Aquatic products, high performance liquid chromatography, Albendazole and its metabolites

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  前言 1
 1.2 阿苯达唑及其主要代谢物检测技术的国内外发展现状 2
2. 实验部分 5
 2.1  试剂和材料 5
 2.2  仪器设备 5
 2.3  实验操作方法 6
3. 结果与讨论 8
 3.1   样品前处理条件的选择 8
 3.2   色谱条件的选择 8
 3.3   精密度和准确度 14
 3.4   方法的灵敏度和检测限 19
4．总结与展望 20

致谢 21
参考文献 22

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