蚕沙叶绿素铁钠盐的制备研究

论文编号:SW039  论文字数:10642,页数:27

摘 要: 本论文利用桑蚕的排泄物蚕沙来制取纯天然叶绿素铁钠，并对其制备制备过程中的提取、皂化和铁代过程进行了最优条件的研究。在叶绿素提取工序中，对微波辐射时间、提取温度、提取时间和提取剂用量做了单因素实验，结果表明，在微波处理90s后，用35ml的95%乙醇(相对于5g干蚕沙)在水浴60℃的情况下提取60min为最佳提取条件。在叶绿素皂化工序中，对碱用量、皂化温度和皂化时间三个因素做了正交试验(糊状叶绿素与NaOH溶液的体积比为4:1)，结果表明，用5.0%NaOH溶液在水浴温度为60℃下皂化50min为最佳皂化条件。在对铁代工序中，对溶液pH值、温度和铁代时间三因素做正交试验(加入的10%FeSO4溶液的体积是所得皂化液体积的四分之一)，结果表明，铁代时将皂化溶液的pH值调为3，水浴温度为60℃,铁代处理60min时铁代反应最完全。

关键词：蚕沙；叶绿素；叶绿素铁钠

Study On Preparation Of
Sodium-Iron-Chlorophylln From Silkworm Feces
Abstract: In this thesis, we studied preparation of natural water-soluble Sodium-Iron-Chlorophylln with silkworm excrement from ilkworm. And we studied the optimal conditions of a number of major processes which were extraction, saponification and Iron substitution. In chlorophyll extraction processes, we made Single-factor experiments about some factors which were radiation time, extraction temperature, extraction time, extract dosage. The results showed that the optimum extraction condition was radiation 90s, 60℃, 35ml 95% ethanol(to 5g silkworm excrement as the standard), and 60℃ water bath. In saponification process(the volume ratio of Chlorophyll paste with NaOH solution is 4:1), we made orthogonal about three factors which were base amount, saponification temperature and saponification time. The results showed that the optimum extraction condition is 5.0% NaOH solution, 50min of saponification and 60℃ water bath. In Iron substitution process(the volume ratio of Saponification solution with 10% FeSO4 solution is 4:1), we made orthogonal about three factors which were pH value, temperature and Iron substitution time. The results showed that the optimum extraction condition was pH value 3, 60min of Iron substitution time and 60℃ water bath. 

Keywords: Silkworm excrement; Chlorophyll; Sodium-Iron- Chlorophylln

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  前言 1
 1.2  概述 1
 1.3 叶绿素铁钠盐的制备原理 5
 1.4 叶绿素铁钠盐制备的工艺路线 6
 1.5 课题研究的意义 7
2. 实验部分 8
 2.1 原理、试剂与仪器 8
 2.2 叶绿素提取条件优先 9
 2.3 叶绿酸钠的制备 12
 2.4 叶绿素铁钠的制备 14
3. 结果与讨论 16
 3.1 产率与转化率 16
 3.2 吸收曲线的测定 16
 3.2 红外光谱图 17
4．总结与展望 19
致谢 21
参考文献 22

相关论文