栝楼籽中活性成分分离及分析方法研究

论文编号:HG073  论文字数:9586,页数:25

 摘　要：随着科学技术的发展,近些年发现栝楼具有很好的医疗作用，因此,国内需求量和出口量增长很快,显示出良好的发展势头。我国是栝楼传统主产国之一,历来国际信誉较高,只要好种植区域,并注意用生物防治法防病治虫,限制农药的使用种类、使用量以及使用时间,并及时作好商品检验和监督,人工栽培栝楼的生产前景是十分广阔的。实验对栝楼籽仁油脂的提取以及油脂的酸价、皂化值和角鲨烯含量进行了分析和测定。结果表明:栝楼籽仁酸价( KOH)为19.23mg/g，皂化值( KOH)为204.84mg/g,角鲨烯含量约为0.1%。确立色谱条件为：色谱柱型号：Hyperil ODSe，流动相为甲醇/乙腈:60/40、检测波长为203nm、流速为2ml/min、柱温为40℃。
 
 关键词：角鲨烯；栝楼籽；成分分析；高效液相色谱
 
 
Separation of Active Components and Analysis Method of Trichosanthes Kiriowii
Abstract : Along with the development of trichosanthes kirilowii in recent years, trichosanthes kirilowii is found good medical effects, therefore, the domestic demand and output grows fast, shows a good development momentum. China is one of the trichosanthes kirilowii traditional production countries with high international prestige. As long as we choose the good planting area and pollution prevention with biological pesticide, limit pest prevention of species, usage and using time, make timely commodity inspection and supervision, the prospect of production of artificial cultivation trichosanthes kirilowii will be very wide.Trchosanthes kiriowii Maxim seed was used as material. Itcomponent s and it s oil properties were studied. The acidvalue( KOH) is 19.23mg/g,saponificationvalue(KOH) is 204.84mg/g, Squalene is 0.1%.The HPLC conditions are the best:column:Hyperil ODSe;
mobile phase，V(acetonitrile)/V（methano1）=60/40;detection wavelength:203nm;
flow rate,2ml／min;tmperature:40℃

Keywords : Squalene;Trchosanthes kiriowii Maxim;Component analysis;HPLC

目 录

1  绪论 1
 1.1   栝楼的特性 1
 1.2  栝楼的食用特点和价值 1
 1.3  栝楼的药理作用 2
 1.4   栝楼的经济效益 3
 1.5   栝楼的开发前景 3
 1.6  角鲨烯 4
 1.7  栝楼籽油的制备法 6
 1.7.1索氏提取法 6
 1.7.2压榨法 6
 1.8  高效液相色谱分析法 7
2  实验部分 8
 2.1  实验原料、试剂与仪器 8
 2.2  栝楼籽仁中粗脂肪的含量测定 8
 2.3  酸价的测定 9
 2.4  皂化值的测定 9
 2.5  色谱条件 10
 2.6. 用螺旋榨油机榨油 10
3  结果和讨论 11
 3.1   标准曲线的绘制 11
 3.2  未经水化脱胶的油脂中角鲨含量测定 13
 3.3  经水化脱胶的油脂中角鲨烯含量测定 14
4  总结与展望 18
致  谢 19
参考文献 20

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