N-苄基壳聚糖衍生物的制备及其应用研究

论文编号:HG067  论文字数:10844,页数:27

 摘 要：本文以苯甲醛和壳聚糖为原料通过席夫碱反应并还原合成N-苄基壳聚糖衍生物，用傅立叶变红外光谱对N-苄基壳聚糖进行了红外表征。通过糠醛比色法测定衍生物的吸光度，发现N-苄基壳聚糖衍生物的胆酸结合能力比未经化学修饰的壳聚糖要高。利用Feton试剂产生羟基自由基，采用水杨酸显色法测定N-苄基壳聚糖衍生物的羟基自由基OH·清除能力，结果显示其有一定的清除能力。利用邻苯三酚自氧化法测定N-苄基壳聚糖衍生物对超氧自由基O2-·的清除能力，结果显示也具有一定清除能力，同时探讨了N-苄基壳聚糖衍生物的溶解性及浓度对清除能力的影响。N-苄基取代改性使浸湿效果和溶解性有了一定的改善，使清除自由基能力提高。但减少了NH2，会使清除自由基能力下降，因此N-苄基壳聚糖衍生物清除自由基能力不高。
 
 关键词：壳聚糖；N-苄基壳聚糖衍生物；胆酸结合能力；自由基清除能力

Study on the Preparation and Application of N-benzyl Chitosan Derivatives
Abstract: This paper introduce the synthesis of N-benzyl derivative through benzaldehyde and chitosan Schiff reaction and was characterized by IR. Determination of furfural derivatives by absorbance, we found N-benzyl derivatives of bile acid binding capacity of chitosan was higher than chitosan. Though using Feton reagent of hydroxyl radical and the color determination of salicylic acid, we tested N-benzyl chitosan derivatives of the hydroxyl radical OH-scavenging ability, the results show that it is have scavenging ability. Use of pyrogallol autoxidation method, we tested N-benzyl chitosan derivatives on O2-superoxide free radical scavenging, the results show also has scavenging, and we discussed the N-benzyl chitosan derivatives solubility and concentration on the scavenging effect. The preparation of N-benzyl modified improved solubility , thus enhancing the free radical scavenging. However, a decrease of NH2, the free radical scavenging ability of thus N-benzyl chitosan derivatives was lower.
Key words: Chitosan; N-benzyl chitosan derivatives; Bile acid binding capacity; Free radical scavenging capacity

目  录
中文摘要 I
英文摘要 II
目  录 III
1  绪论 1
1.1  壳聚糖简介 1
1.2  壳聚糖及衍生物的应用 1
1.4  壳聚糖及其衍生物改性方法及性能 3
1.5  壳聚糖的N-苄基化反应（席夫碱反应） 4
1.6   N-苄基壳聚糖衍生物表征 4
1.7   N-苄基壳聚糖衍生物的生物活性 5
1.8  实验思路 6
2  实验部分 8
2.1  材料试剂与设备 8
2.2  实验步骤 8
2.3  结果与讨论 12
2.4  结论与展望 17
致 谢 19
参考文献 20

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